Production of active poly- and oligosaccharidic fractions from Ulva sp. By combining Enzyme-Assisted Extraction (EAE) and depolymerization

TitreProduction of active poly- and oligosaccharidic fractions from Ulva sp. By combining Enzyme-Assisted Extraction (EAE) and depolymerization
Type de publicationArticle
Année de publication2019
Langueanglais
Titre de la revueMetabolites
Mention d'éditeurMDPI
Volume9
Numéro9
Pagination182
Date de publication12/09
Auteur(s)Fournière, M., Latire T., Lang M., Terme N., Bourgougnon N. et Bedoux G.
Résumé

Data on fractionation and depolymerization of the matrix ulvan polysaccharides, and studies on the biological activities on skin cells, are very scarce. In this work, crude ulvans were produced by using EAE (enzyme-assisted extraction) and compared to maceration (an established procedure). After di erent fractionation procedures—ethanolic precipitation, dialysis, or ammonium sulfate precipitation—the biochemical composition showed that EAE led to an increased content in ulvans. Coupling EAE to sulfate ammonium precipitation led to protein enrichment. Oligosaccharides were obtained by using radical depolymerization by H2O2 and ion-exchange resin depolymerization. Sulfate groups were partially cleaved during these chemical treatments. The potential bioactivity of the fractions was assessed using a lipoxygenase inhibition assay for anti-inflammatory activity and a WST-1 assay for human dermal fibroblast viability and proliferation. All ulvans extracts, poly- and oligosaccharidic fractions from EAE, expanded the fibroblast proliferation rate up to 62%. Our research emphasizes the potential use of poly- and oligosaccharidic fractions of Ulva sp. for further development in cosmetic applications.

DOI10.3390/metabo9090182