Production of Poly- and Oligosaccharidic Fractions from Macroalga Ulva sp.: Regulation of Extracellular Matrix Renewal in Human Skin Fibroblasts

TitreProduction of Poly- and Oligosaccharidic Fractions from Macroalga Ulva sp.: Regulation of Extracellular Matrix Renewal in Human Skin Fibroblasts
Type de publicationCommunications avec actes
Année de publication2021
LangueAnglais
Titre de la Conférence/colloquethe 7th conférence of international society for applied phycology
Titre des actes ou de la revueActes du 7ème colloque
Auteur(s)Fournière, M., Bedoux G., Bourgougnon N., Lebonvallet N. et Latire T.
Résumé

Ulva sp., a green macroalga mostly composed of ulvans (matrix phase water-soluble sulfated polysaccharides), is also known to produce other compounds of interest such as proteins [1]. Currently, only few studies investigate the effects of Ulva components on cutaneous system for cosmetics applications. Previous study on ulvans extraction and purification from hot water extraction (maceration) showed an increase in hyaluronan production in human dermal fibroblast [2]. In a previous study we performed EAE (enzyme-assisted extraction) using endo-protease Protamexâ on dried Ulva sp. to produce crude ulvans. Fractionation (ethanolic precipitation, ammonium sulfate precipitation and dialysis) and depolymerization procedures (radical depolymerization by H2O2 and ion-exchange resin depolymerization) were investigated to produce poly- and oligosaccharidic fractions. We demonstrated that these fractions exhibited anti-inflammatory activity (lipoxygenase inhibition of 50% at 500 μg/mL) and improved human dermal fibroblast viability and proliferation up to 62% [3]. Fibroblasts that are the main cellular population of dermis, maintain the extracellular matrix (ECM) homeostasis with a balance between matrix components (collagens, matrix metalloproteinase, glycosaminoglycans, etc). In our ongoing research, we investigated the potential biological activity of Ulva sp. fractions on extracellular matrix renewal of human skin fibroblasts, considering both anabolic and catabolic pathways. The proteomic (on extra- and intracellular proteins) and transcriptomic (mRNA steady-state level) analyses showed that fractions stimulated the synthesis of ECM components such as collagen (type I up to 280%), matrix metalloproteinases (MMP-1 up to 260%), non-sulphated and sulphated glycosaminoglycans (+46% and +62% respectively). Our research emphasizes for the first time the potential use of crude and depolymerized ulvans, using green technology, for dermo-cosmetic applications in skin anti-aging strategies. Further investigations will be needed to assess whether the molecules act as signaling molecules with cell mediation or matrix action.